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Comparison of young (8 weeks) and aged (110 weeks) CD11C+ splenic dendritic cells from C57BL/6 mice

ABSTRACT: Total RNA was isolated from FACS purified CD11c+ splenic DC populations isolated from young (8 weeks old, n=3) and aged (110 weeks old, n=3) mice, that had been injected with Poly I:C for 4h previously. Microarray analysis was performed with the Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. The arrays were hybridized, washed and scanned according to the standard Affymetrix protocol. The gene chip tests were performed by professional staff of Shanghai Biochip Company, PRC. Gene expression profiles and functional gene set enrichment analysis demonstrate that DC isolated aged mice are impaired in their ability to activate Natural Killer (NK) cells. The study is submitted for publication in June 2014.

INSTRUMENT(S): Affymetrix, Agilent Bioanalyzer

ORGANISM(S): Mus musculus

SUBMITTER: Tamara Tilburgs

PROVIDER: E-MTAB-2863 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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