Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

30 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional signature of unipotent luminal progenitors of the mouse mammary gland

ABSTRACT: We report here the transcriptional signature of Notch1-expressing (GFP positive) luminal mammary progenitors compared to total luminal cells (GFP negative). For each condition, 3 biological replicates were performed, by pooling the mammary glands from two mice/replicate.

ORGANISM(S): Mus musculus

SUBMITTER: Silvia Fre

PROVIDER: E-MTAB-2897 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Notch activation in the mouse mammary luminal lineage leads to ductal hyperplasia and altered partitioning of luminal cell subtypes

Project description:In this study, we express an activated form of Notch1 in the mouse mammary luminal lineage and analyse the consequences for tumour formation and the transcriptomic landscape in the luminal lineage. Simultaneous conditional activation of a Notch1 intracellular domain (Notch1 ICD) and EGFP in the luminal lineage was achieved by removal of a stop cassette by CRE-recombinase expression from the whey acidic protein (WAP) promoter. Gene expression differences were analysed by single cell RNA-sequencing.

2020-09-20 | GSE137795 | GEO

GATA-3 maintains luminal cell differentiation in the mammary gland and in breast cancer

2006-10-24 | GSE5602 | GEO

Transcriptome analysis of luminal and basal cell subpopulations in the lactating versus pregnant mammary gland

Project description:To identify genes specifically expressed in lactating mammary glands, the gene expression profiles of luminal and basal cells from different developmental stages were compared. Comparison of gene expression in luminal and basal cells harvested from the mammary glands of virgin, 18.5 day pregnant and 2 day lactating mice (2 mice per stage).

2015-01-19 | E-GEOD-60450 | biostudies-arrayexpress

GATA-3 maintains luminal cell differentiation in the mammary gland and in breast cancer

Project description:We have identified GATA-3 as a critical regulator of luminal cell differentiation in the mammary gland. Acute loss of GATA-3 in the adult mammary gland leads to an expansion of an undifferentiated luminal epithelium and the formation of a multi-layered epithelium. Here we report microarray analysis of mammary glands that have undergone acute loss of GATA-3 Adult GATA-3flox/flox; WAP-rtTA-Cre and GATA-flox/+; WAP-rtTA-Cre mice were administered doxyxcline for 5 days and their mammary glands harvested. Total RNA was extracted by the Trizol method. Het mammary gland total RNA was labeled with Cy5 while Null mammary gland total RNA was labeled with Cy3. Microarray hybridization was performed on spotted oligonucleotide microarrays with 38,000 features. Lowess print-tip normalization and analysis was performed on the Acuity software package (V 4.0)

2010-07-01 | E-GEOD-5602 | biostudies-arrayexpress

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [02_citeseq_shTRPS1_mmg]

Project description:The GATA transcription factor TRPS1 is a context-dependent oncogene in breast cancer. In the mammary gland, TRPS1 activity is restricted to the luminal population and sustains the initial branching of the mammary ducts at puberty as well as the lactogenic differentiation during pregnancy. Its function in the resting mammary gland however, remains unclear. To evaluate whether it could be a target for cancer therapy, we investigated TRPS1 function in the healthy resting mammary gland using a conditional ubiquitous depletion mouse model where long-term depletion does not affect fitness. we show that TRPS1 activity is critical to maintain a functional luminal progenitor compartment and that this involves the repression of the SRF/MRTF and the YAP/TAZ transcriptional program. Mechanistically, this repression involves Krt8-dependent RhoA modulation. Our work uncovers a hitherto undisclosed function of TRPS1 in luminal progenitors which is intrinsically linked to mechanotransduction in the mammary gland. It also provides new insights into the oncogenic functions of TRPS1 since luminal progenitors are likely the cells of origin of many breast cancers.

2024-05-24 | GSE243508 | GEO

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [01d_bulk_rna_seq_lum_prog_vs_mature]

2024-05-24 | GSE243507 | GEO

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [01b_bulk_rna_seq_luminal_vs_basal]

2024-05-24 | GSE243505 | GEO

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [01a_bulk_rna_seq_PCX_Cre_shTRPS1]

2024-05-24 | GSE243504 | GEO