Genome-wide ribosome profiling to identify regulatory targets of small RNA RyhB in E.coli
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ABSTRACT: The effects of RyhB expression were examined by Ribo-seq and RNA-seq after 10 min to avoid indirect effects. Expression of RyhB was induced by arabinose from cells carrying pBAD-ryhB plasmid. The RyhB expression was confirmed by real-time PCR. As a control, cells with vector pNM12 were grown and induced. The cells were pulverized and total mRNAs were extracted from the pulverized cells and processed for Ribo-seq and RNA-seq.
INSTRUMENT(S): Illumina HiSeq 2500, Retsch MM400
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655
SUBMITTER: Jing Wang
PROVIDER: E-MTAB-2903 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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