3' UTR length and mRNP composition determine endocleavage efficiencies at termination codons

ABSTRACT: We aimed to study the cleavage sites of nonsense-mediated mRNA decay (NMD) substrates. Therefore, we depleted the major exoribonuclease XRN1 in human cell culture, which degrades the 3' fragments generated by SMG6-mediated endonucleolytic cleavage. Different reporter mRNAs or endogenous NMD targets were investigated and the 3' fragments were cloned, amplified and subsequently subjected to high throughput sequencing.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Volker Boehm 

PROVIDER: E-MTAB-2907 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress