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CPSF-160 iCLIP

ABSTRACT: We performed iCLIP of CPSF-160- GFP as CPSF160 was believed to be the main RNA-binding subunit of CPSF. To this end, we used a stable HeLa cell line that expresses GFP-CPSF160 in a doxycycline (Dox)-dependent manner. After GFP-CPSF160 was induced, we irradiated the cells with UV-C (wavelength=254nm) to induce protein-RNA crosslinks and immunoprecipitated the complex with a GFP antibody.

INSTRUMENT(S): iCLIP materials, Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Ina Huppertz

PROVIDER: E-MTAB-2939 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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