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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic analysis of hearts from high fat diet fed mice with over expression or knock down of microRNA-322

ABSTRACT: Cardiac function of mice fed with a 60%fat diet (high fat diet, HFD) for 16 weeks was analyzed. Hearts showed an alteration in ejection fraction and an increase in left ventricular en systolic diameter. However mice injected with an AAV overexpressing microRNA-322 (miR-322) were protected: ejection fraction was less decreased and LVESD less increased than in animals treated with an AAV control or an AAVsponge anti miR-322 (which decrease miR-322 availability and action in the heart). To better understand globla changes induced by miR-322 modulation in the heart, a transcriptomic analysis was performed to compare gene expression in three hearts from HFD+AAVmiR-322 mice (showing cardiac protection) and gene expression in three hearts from HFD+AAvsponge (were miR-322 action is decreased and showing the most altered cardiac function).

ORGANISM(S): Mus musculus

SUBMITTER: Anne-Marie Lompre

PROVIDER: E-MTAB-2966 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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