Unknown,Transcriptomics,Genomics,Proteomics

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Gene Expression Patterns in Potato Roots Associated with Greater Plant Growth at Low Phosphate Concentrations in the Rhizosphere

ABSTRACT: There are both financial and environmental reasons for optimising the use of phosphorus (P) fertilisers in potato production. One strategy to effect this is to develop genotypes with greater P use efficiency (PUE), which is generally defined as yield divided by the P available to the crop. In this article we report that potato (Solanum tuberosum L.) genotypes differ in their PUE, expressed both as yield in the field and as shoot biomass when grown hydroponically. When grown hydroponically, PUE was strongly correlated with P uptake efficiency (PUpE, defined as shoot P content), but not with physiological P utilisation efficiency (PUtE, defined as shoot biomass divided by shoot P content). Gene expression in roots differed between potato genotypes, and changed with P supply. A common transcriptional response to reduced P supply was determined in roots of four potato genotypes. This response comprised genes encoding enzymes involved in increasing rhizosphere P availability, phosphate transport, P-sparing metabolism, replacement of phospholipids in cell membranes, and various transcription factors. A specific transcriptional response associated only with roots of genotypes with high yields in the absence of P-fertiliser application in the field (Maris Piper and Stirling) was also identified. Genes involved in this response encoded various transcription factors, proteins facilitating water, solute and auxin transport, and enzymes involved in polyamine metabolism and the biosynthesis of tropane alkaloids.

ORGANISM(S): Solanum tuberosum

SUBMITTER: Pete Hedley

PROVIDER: E-MTAB-2990 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Low phosphate concentrations are frequently a constraint for maize growth and development, and therefore, enormous quantities of phosphate fertilizer are expended in maize cultivation, which increases the cost of planting. Low phosphate stress not only increases root biomass but can also cause significant changes in root morphology. Low phosphate availability has been found to favor lateral root growth over primary root growth by dramatically reducing primary root length and increasing lateral root elongation and lateral root density in Arabdopsis. While in our assay when inbred line Q319 subjected to phosphate starvation, The numbers of lateral roots and lateral root primordia were decreased after 6 days of culture in a low phosphate solution (LP) compared to plants grown under normal conditions (sufficient phosphate, SP), and these differences were increased associated with the stress caused by phosphate starvation. However, the growth of primary roots appeared not to be sensitive to low phosphate levels. This is very different to Arabidopsis. To elucidate how low phosphate levels regulate root modifications, especially lateral root development, a transcriptomic analysis of the 1.0-1.5 cm lateral root primordium zone (LRZ) of maize Q319 treated after 2 and 8 days by low phosphate was completed respectively. The present work utilized an Arizona Maize Oligonucleotide array 46K version slides, which contained 46,000 maize 70-mer oligonucleotides designated by TIGR ID, and the sequence information is available at the website of the Maize Oligonucleotide Array Project as the search item representing the >30,000 identifiable unique maize genes (details at http://www.maizearray.org). Keywords: low phosphate, Lateral Root Primordium Zone, maize Two-condition experiment, low phosphate treated lateral root primordium zone of maize root vs. normal cultrued lateral root primordium zone. Biological replicates: 9 control, 9 treated, independently grown and harvested. One replicate per array.

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Gene Expression Patterns in Potato Roots Associated with Greater Plant Growth at Low Phosphate Concentrations in the Rhizosphere

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