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Whole transcriptome sequencing of splenic influenza-specific CD8+ T cells in wt and Notch1/2ko mice

ABSTRACT: To more specifically determine whether the TEC program is controlled by Notch, we generated molecular definitions of TECs and MPCs by whole transcriptome sequencing of sorted KLRG1-CD127+ and KLRG1+CD127- H-2 Db-NP specific CD8+ T cell populations from wild-type mice. These definitions were used to analyze data derived from RNAseq of splenic H-2 Db-NP+CD8+ T cells from WT and Notch1-2-KO mice 10 days post-infection with A/HKx31.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Christina Helbig

PROVIDER: E-MTAB-2999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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PD-1 blockade potentiates neoadjuvant chemotherapy in NSCLC via increasing CD127+ and KLRG1+ CD8 T cells

Project description:The combination of PD-1 blockade with neoadjuvant chemotherapy (NAC) has achieved unprecedented clinical success in non-small-cell lung cancer (NSCLC) compared to NAC alone, but the underlying mechanisms by which PD-1 blockade augments the effects of chemotherapy remain incompletely elucidated. Single-cell RNA sequencing was performed on CD45+ immune cells isolated from surgically resected fresh tumor tissues of seven NSCLC patients receiving NAC or neoadjuvant pembrolizumab and chemotherapy (NAPC). Multiplex fluorescent immunohistochemistry was performed on paired FFPE tissues before and after NAC or NAPC from 65 resectable NSCLC patients, and results were validated with GEO dataset. NAC resulted in an increase only of CD20+ B cells, whereas NAPC increased the infiltration of CD20+ B cells, CD4+ T cells, CD4+CD127+ T cells, CD8+ T cells, CD8+CD127+ and CD8+KLRG1+ T cells. Synergistic increase in B and T cells promotes favorable therapeutic response after NAPC. Spatial distribution analysis discovered that CD8+ T cells and their CD127+ and KLRG1+ subsets were in closer proximity to CD4+ T/CD20+ B cells in NAPC versus NAC. GEO dataset validated that B-cell, CD4, memory and effector CD8 signatures correlated with therapeutic responses and clinical outcomes. The adding of PD-1 blockade to NAC promoted anti-tumor immunity through T and B cells recruitment in the tumor microenvironment and induced tumor-infiltrating CD8+ T cells skewed toward CD127+ and KLRG1+ phenotypes，which may be assisted by CD4+ T cells and B cells. Our comprehensive study identified key immune cell subsets exerting anti-tumor responses during PD-1 blockade therapy and that may be therapeutically targeted to improve upon existing immunotherapies for NSCLC.

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ASC controls T-bet-dependent CTL differentiation and defines transplant outcome independent of the inflammasome

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| PRJNA954049 | ENA

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2022-11-03 | E-MTAB-11397 | biostudies-arrayexpress

ASC controls T-bet-dependent CTL differentiation and defines transplant outcome independent of the inflammasome

2016-04-01 | GSE78755 | GEO

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