Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

The outer mitochondrial membrane AAA ATPase AtOM66 affects cell death and pathogen resistance in Arabidopsis thaliana

ABSTRACT: 3 week old AtOM66 overexpression and knockout plants were compared to wild type Col-0 plants during a drought stress time course. Samples were taken 2 days after last watering (untreated), 9 days (early drought) and 11 days (late drought).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Olivier Van Aken

PROVIDER: E-MTAB-3044 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The mitochondrial outer membrane AAA ATPase AtOM66 affects cell death and pathogen resistance in Arabidopsis thaliana.

Zhang Botao B Van Aken Olivier O Thatcher Louise L De Clercq Inge I Duncan Owen O Law Simon R SR Murcha Monika W MW van der Merwe Margaretha M Seifi Hamed Soren HS Carrie Chris C Cazzonelli Christopher C Radomiljac Jordan J Höfte Monica M Singh Karam B KB Van Breusegem Frank F Whelan James J

The Plant journal : for cell and molecular biology 20141101 4

One of the most stress-responsive genes encoding a mitochondrial protein in Arabidopsis (At3g50930) has been annotated as AtBCS1 (cytochrome bc1 synthase 1), but was previously functionally uncharacterised. Here, we show that the protein encoded by At3g50930 is present as a homo-multimeric protein complex on the outer mitochondrial membrane and lacks the BCS1 domain present in yeast and mammalian BCS1 proteins, with the sequence similarity restricted to the AAA ATPase domain. Thus we propose to ...[more]

PMID: 25227923

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Project description:Switchgrass plants were grown in a Sandwich tube system to induce gradual drought stress by withholding watering. After 29 days, leaf photosynthetic rate decreased significantly, compared to the control plants which were watered regularly. The drought-treated plants recovered to the same leaf water content after three days of re-watering. Root tip (1cm basal fragment, designated as RT1 hereafter) and the elongation/maturation zone (the next upper 1 cm tissue, designated as RT2 hereafter) tissues were collected at the 29th day of drought stress treatment, (named SDT for severe drought treated), one (D1W) and three days (D3W) of re-watering. The tandem mass tags mass spectrometry-based quantitative proteomics analysis was performed to identify the proteomes, and drought-induced differentially expressed proteins (DEPs). From RT1 tissues, 6,156, 7,687 and 7,699 proteins were quantified, and 296, 535 and 384 DEPs were identified in the SDT, D1W and D3W samples, respectively. From RT2 tissues, 7,382, 7,255 and 6,883 proteins were quantified, and 393, 587 and 321 proteins DEPs were identified in the SDT, D1W and D3W samples. Between RT1 and RT2 tissues, very few DEPs overlapped at SDT, but the number of such proteins increased during the recovery phase. A large number of hydrophilic proteins and stress-responsive proteins were induced during SDT and remained at a higher level during the recovery stages. A large number of DEPs in RT1 tissues maintained the same expression pattern throughout drought treatment and the recovery phases. The DEPs in RT1 tissues were classified in cell proliferation, mitotic cell division, and chromatin modification, and those in RT2 were placed in cell wall remodeling and cell expansion processes. This study provided information pertaining to root zone-specific proteome changes during drought and recover phases, which will allow us to choose the proteins (genes) as better defined targets for developing drought tolerant plants.

Dataset Information

The outer mitochondrial membrane AAA ATPase AtOM66 affects cell death and pathogen resistance in Arabidopsis thaliana

Publications

The mitochondrial outer membrane AAA ATPase AtOM66 affects cell death and pathogen resistance in Arabidopsis thaliana.

Similar Datasets

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