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Unknown,Transcriptomics,Genomics,Proteomics

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Microarray analysis of DNA methylation in metastatic versus non metastatic breast cancers

ABSTRACT: 19 non-metastatic breast cancers (controls) and 19 breast cancers showing metastases or metastatic relapse within 5 years (cases) were extracted from a multi-institutional case series of 123 breast cancer patients . Cases and controls were analyzed for DNA methylation over 56 genes by the MethDet assay [1]using a dedicated microarray (MethDet56). 1. Levenson VV, Melnikov AA: The MethDet: a technology for biomarker development. Expert Rev Mol Diagn 2011, 11:807-812.

INSTRUMENT(S): GenePix 4000B Microarray Scanner (Molecular Devices).

ORGANISM(S): Homo sapiens

SUBMITTER: Emanuela Guerra

PROVIDER: E-MTAB-3153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A seven-gene CpG-island methylation panel predicts breast cancer progression.

Li Yan Y Melnikov Anatoliy A AA Levenson Victor V Guerra Emanuela E Simeone Pasquale P Alberti Saverio S Deng Youping Y

BMC cancer 20150519

<h4>Background</h4>DNA methylation regulates gene expression, through the inhibition/activation of gene transcription of methylated/unmethylated genes. Hence, DNA methylation profiling can capture pivotal features of gene expression in cancer tissues from patients at the time of diagnosis. In this work, we analyzed a breast cancer case series, to identify DNA methylation determinants of metastatic versus non-metastatic tumors.<h4>Methods</h4>CpG-island methylation was evaluated on a 56-gene canc ...[more]

PMID: 25986046

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