Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

44 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of CtBP1 knockdown in SCC25 cells

ABSTRACT: Cells were transfected with siRNA targetting CtBP1, or a non-targetting control siRNA. mRNA was extracted for gene expression analysis 48 hours after transfection

INSTRUMENT(S): RNAeasy (Qiagen), Ambion WT, Ambion WT / Affymetrix

ORGANISM(S): Homo sapiens

SUBMITTER: Jeremy Blaydes

PROVIDER: E-MTAB-3295 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Effect of knockdown of CtBP proteins on gene expression in MCF7 breast cancer cells, under normoxia and hypoxia

Project description:Regulation of gene expression by the CtBP family of NADH-sensitive transcriptional regulators, in MCF7 cells under normoxia and hypoxia. To determine the effect of CtBP knockdown on gene expression in MCF7 we transfected cells with an siRNA (5′-GGGAGGACCUGGAGAAGUUdTdT-3′/3′-dTdTCCCUCCUGGACCUCUUCAA-5′, obtained from Ambion) targetting both CtBP1 and CtBP2 (versus control siRNA). After 48 hours cells were either transferred to a hypoxic chamber (1% oxygen), or maintained in normoxia, for 18 hours.

2021-02-01 | GSE111259 | GEO

Analysis of gene expression signatures for Met knockdown by siRNA

Project description:To comprehensively detect differential gene expression between ovarian cancer cell lines (ES-2) transfected with fibroblast-derived exosomes only or Met targetting siRNA containing fibroblast-derived exosomes, total RNA was extracted and cDNA microarray analyses were performed.

2023-10-15 | GSE159573 | GEO

The effect of knockdown of amphiregulin or SGK3 on global gene expression in letrozole resistant breast cancer cells

Project description:LET-R cells were reversely transfected with siRNA negative control, AREG siRNA or SGK3 siRNA for 48 h, and then harvested for RNA sequencing.

2020-04-23 | GSE148800 | GEO

Alternative splicing signatures in apoptosis related genes of human HeLa cells with RNAi targeting p68 RNA helicase-DDX5

Project description:Alternative splicing profiling of apopotosis related genes in human HeLa cells (cervical cancer cell line) transfected with a plasmid expressing shRNAs targetting p68 helicase (DDX5, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5) cloned into the pSuper expression vector compared to empty vector. Keywords: treated vs. untreated comparison; alternative splicing Two-condition experiment, where the p68 DDX5 gene product levels was inhibited by siRNA transfection and compared to transfection with the negative control (scrambled siRNA). Biological replicates: 2, all independently grown and harvested. A dye swapping technical duplicate was performed for each biological replicate. Samples were hybridized onto a 44290 feature array designed by ExonHit to detect splicing events in apopotosis related genes and manufactured by Agilent using in situ synthesis of oligonucleotides by SurePrint technology.

2011-01-21 | E-GEOD-20010 | biostudies-arrayexpress

LSD1 knockdown in 3T3-L1 preadipocytes

Project description:Obesity is often associated with a low-grade systemic inflammation state that contributes to the development of insulin resistance and atherosclerotic complications. This is usually coupled with increased macrophage infiltration in the adipose tissue and a defect in adipocyte differentiation that results in accumulation of hypertrophic fat cells characterized by a deregulated pattern of adipokine expression. Here we show that knockdown of histone demethylase lsd1 in 3T3-L1 preadipocytes results in defective adipogenesis and derepression of an inflammatory program in these cells. The dataset consists of four sample groups: [1] 3T3-L1 preadipocytes (passage 19) transfected with a control scrambled siRNA at 24h after transfection (siC.24h), [2] 3T3-L1 preadipocytes (p.19) transfected with a siRNA directed against LSD1 at 24h after transfection (siLsd1.24h), [3] 3T3-L1 preadipocytes (p.21) transfected with a control scrambled siRNA at 48h after transfection (siC.48h), and [4] 3T3-L1 preadipocytes (p.21) transfected with a siRNA directed against LSD1 at 48h after transfection (siLsd1.48h). The 24h sample groups (siC.24h and siLsd1.24h) consist of two biological replicate samples; the 48h sample groups (siC.48h and siLsd1.48h) consist of three biological replicate samples. Each sample was hybridized to a separate array, for a total of ten arrays.

2013-12-31 | E-GEOD-25423 | biostudies-arrayexpress

Effect of YBX1 knockdown on gene expression of differentiated C3H10T1/2 brown adipocytes

Project description:To investigate the function of YBX1 in the regulation of brown adipose aging, differentiated C3H10T1/2 brown adipocytes were transfected with specific siRNAs targeting YBX1 or control siRNA. Total RNA was extracted at 48h after transfection to performed gene expression profiling analysis by high throughput RNA-seq.

2024-01-04 | GSE222103 | GEO

Chromatin accessiblity of 3T3-L1 cells with Kdm5c knockdown

Project description:One day prior to confluency, non-targeting (scramble control) siRNA or Kdm5c siRNA were transfected into 3T3-L1 cells and chromatin structure was assessed by ATAC-seq at 24hr, 48hr, and 72 hr post transfection.

2020-09-07 | GSE147994 | GEO

High-throughput sequencing of 3T3-L1 cells with Kdm5c knockdown

Project description:One day prior to confluency, non-targeting (scramble control) siRNA or Kdm5c siRNA were transfected into 3T3-L1 cells and gene expression was assessed by RNA-seq at 24hr, 48hr, and 72hr post transfection.

2020-09-07 | GSE147997 | GEO

Gene expression altered by HOTAIR knockdown in MCF-7-TNR

Project description:The human HOTAIR-specific siRNA and control siRNA were transfected into MCF-7-TNR cells. RNA was collected at 72 hrs after transfection. RNA-SEQ was carried out to profile the gene expression altered by HOTAIR knockdown.

2019-01-11 | GSE124934 | GEO

UPF1 knockdown in differentiating human myoblasts

Project description:Human myoblast cell line 54-1 is transfected with either a srambled control siRNA or siRNA against UPF1. Two days after transfection, cell were induced to differentiate by changing grow meida to differentiation media. 2 days after induction of differentiation, cells are collected for extraction of RNA.

2017-08-31 | GSE87679 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data