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TSAR1 and TSAR2 activate triterpene saponin production in Medicago truncatula

ABSTRACT: We identified two novel transcription factors ‘Triterpene Saponin Activation Regulator’ (TSAR) 1 and 2 that each activate a specific branch of saponin synthesis in M. truncatula. Therefore we generated three independent TSAR1 and three independent TSAR2 overexpression (OE) hairy root lines. As a control we raised three independent hairy root lines expressing a non-functional GUS gene. By qRT-PCR analyses and metabolite profiling of these lines, we found that TSAR1 OE leads to accumulation of non-haemolytic saponins whereas TSAR2 instigates accumulation of haemolytic saponins. To identify additional targets of TSAR1 and TSAR2 we carried out a genome-wide transcript profiling study by RNA-Seq.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Medicago truncatula

SUBMITTER: Jan Mertens 

PROVIDER: E-MTAB-3532 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The bHLH Transcription Factors TSAR1 and TSAR2 Regulate Triterpene Saponin Biosynthesis in Medicago truncatula.

Mertens Jan J   Mertens Jan J   Pollier Jacob J   Vanden Bossche Robin R   Lopez-Vidriero Irene I   Franco-Zorrilla José Manuel JM   Goossens Alain A  

Plant physiology 20151120 1

Plants respond to stresses by producing a broad spectrum of bioactive specialized metabolites. Hormonal elicitors, such as jasmonates, trigger a complex signaling circuit leading to the concerted activation of specific metabolic pathways. However, for many specialized metabolic pathways, the transcription factors involved remain unknown. Here, we report on two homologous jasmonate-inducible transcription factors of the basic helix-loop-helix family, TRITERPENE SAPONIN BIOSYNTHESIS ACTIVATING REG  ...[more]

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