Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profiling by array of Solenopsis invicta (fire ant) newly mated queens to identify genes and gene pathways responding to infection by SINV viruses

ABSTRACT: We performed a whole-genome transcriptomic analysis of pleometrotic queens infected by SINV-1, SINV-2 or co-infected with both viruses, to characterize patterns of gene expression associated with viral infection and identify genes responding differentially to the two viruses. We sampled fire ant queens in Gainesville, Florida, immediately after a mating flight. We arranged them in pairs based on having similar weights (range ±0.2 mg) to allow pleometrotic colony founding. We housed paired queens in nesting tubes in claustral conditions (no food or water) for one month. Thereafter, we collected queens in dry ice, we isolated total RNA from whole bodies and used it to quantify SINV-1 and SINV-2 viruses with real-time PCR. We selected 2 queens that were virus-free as controls, 3 SINV-1-infected, 4 SINV-2-infected and 11 co-infected by both viruses. These samples were processed for microarrays analysis.

INSTRUMENT(S): Qiagen, Life Technologies, Thermo, Ambion, Agilent, Real-time PCR, Ambion, MAUI

ORGANISM(S): Solenopsis invicta

SUBMITTER: Fabio Manfredini

PROVIDER: E-MTAB-3581 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:In many animals living in groups the reproductivestatus of individuals is determined by their social status. In specieswith social hierarchies, the death of dominant individuals typicallyupheaves the social hierarchy and provides an opportunity for subordinateindividuals to improve their social status. Such a phenomenon occursin the monogyne form of the fire ant\emph{, Solenopsis invicta, }wherecolonies typically contain a single wingless reproductive queen, thousandsof workers and hundreds of winged non-reproductive virgin queens.Upon the death of the mother queen, many virgin queens shed theirwings and initiate reproductive development instead of departing ona mating flight. Workers progressively execute almost all of themover the following weeks. The workers base their collective decisionon pheromonal cues associated with the onset of reproductive developmentof the virgin queens which occurs after orphaning. To examine the factors that determine which virgin queens are executed and which survive, we set up artificial competitions between queens from different colonies. Using microarrays, we found that queens from winning colonies showed higher mitochondrial as well as organ development activities 24 hours after orphaning than did queens from colonies that lost the competitions. Furthermore, queens from colonies where queens shed their wings faster after orphaning were more likely to survive competitions. Finally, higher wing shedding speed is linked to higher mitochondrial activity. Eight competitions were initially conducted between queens form pairs of colonies. Six competitions clearly identified one winning colony and one losing colony. We thus have microarray data for six competitions (ie biological replicates), with one winning and one losing colony within each competition. Thus 12 colonies total. For each colony, RNA extracted from five queens was pooled, and hybridized against an unrelated common reference RNA that had been made from a pool of many S. invicta individuals of all castes and developmental stages. Lab work was conducted in blocks (by competition) with randomized order within competition to avoid introducing bias. No technical replication was performed.

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Transcriptional profiling by array of Solenopsis invicta (fire ant) newly mated queens to identify genes and gene pathways responding to infection by SINV viruses

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