Distinctive responses of Mycobacterium smegmatis to exposure of low and high levels of hydrogen peroxide
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ABSTRACT: Log phase cultures (OD600 of 0.8 - 1.0) of M. smegmatis mc2155 were diluted 1:100 into 7H9 media and cultured for approximately 12 hours until the OD600 reached 0.3. Re-inoculated cells were then treated with the indicated concentrations of H2O2 (0.2 and 7 mM) for periods of 30 min and the corresponding total RNAs were isolated and compared by RNA-sequencing to RNA from untreated cells that were prepared simultaneously.
INSTRUMENT(S): Illumina HiSeq 2000
ORGANISM(S): Mycobacterium smegmatis str. MC2 155
SUBMITTER: Xiaojing Li
PROVIDER: E-MTAB-3594 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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