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Detection of potential neuroprotective changes at the molecular, anatomical and physiological level in identified human pyramidal cells in response to brain edema and increased intracranial pressure.

ABSTRACT: Here we show a workflow combining single cell electrophysiology, anatomy and molecular biology in neurons identified in situ to assess mRNA expression at single molecule precision and corresponding functional changes in response to edema and increased intracranial pressure. This combination of methods applied to human pyramidal neurons reveals quantitative changes of known and potential targets in the therapy of brain edema and traumatic brain injury.

ORGANISM(S): Homo sapiens

SUBMITTER: Ágnes Zvara

PROVIDER: E-MTAB-3678 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human neuronal changes in brain edema and increased intracranial pressure.

Faragó Nóra N Kocsis Ágnes Katalin ÁK Braskó Csilla C Lovas Sándor S Rózsa Márton M Baka Judith J Kovács Balázs B Mikite Katalin K Szemenyei Viktor V Molnár Gábor G Ozsvár Attila A Oláh Gáspár G Piszár Ildikó I Zvara Ágnes Á Patócs Attila A Barzó Pál P Puskás László G LG Tamás Gábor G

Acta neuropathologica communications 20160804 1

Functional and molecular changes associated with pathophysiological conditions are relatively easily detected based on tissue samples collected from patients. Population specific cellular responses to disease might remain undiscovered in samples taken from organs formed by a multitude of cell types. This is particularly apparent in the human cerebral cortex composed of a yet undefined number of neuron types with a potentially different involvement in disease processes. We combined cellular elect ...[more]

PMID: 27487831

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