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RNA-seq of poly-A enriched total RNA of brain, liver, kidney, heart and skeletal muscle samples from frog

ABSTRACT: This experiment contains the Xenopus tropicalis subset of data from the experiment E-GEOD-41338 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41338/). mRNA profiles of several organs (brain, liver, kidney, heart, skeletal muscle) in multiple vertebrate species (mouse, chicken, lizard, frog, pufferfish) were generated by deep sequencing using Illumina HiSeq to better understand how species with similar repertoires of protein-coding genes differ so markedly at the phenotypic level.

ORGANISM(S): Xenopus tropicalis

SUBMITTER: Nuno Barbosa-Morais

PROVIDER: E-MTAB-3726 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The evolutionary landscape of alternative splicing in vertebrate species.

Barbosa-Morais Nuno L NL Irimia Manuel M Pan Qun Q Xiong Hui Y HY Gueroussov Serge S Lee Leo J LJ Slobodeniuc Valentina V Kutter Claudia C Watt Stephen S Colak Recep R Kim TaeHyung T Misquitta-Ali Christine M CM Wilson Michael D MD Kim Philip M PM Odom Duncan T DT Frey Brendan J BJ Blencowe Benjamin J BJ

Science (New York, N.Y.) 20121201 6114

How species with similar repertoires of protein-coding genes differ so markedly at the phenotypic level is poorly understood. By comparing organ transcriptomes from vertebrate species spanning ~350 million years of evolution, we observed significant differences in alternative splicing complexity between vertebrate lineages, with the highest complexity in primates. Within 6 million years, the splicing profiles of physiologically equivalent organs diverged such that they are more strongly related ...[more]

PMID: 23258890

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