Dataset Information
RNA-seq analysis of parental (wild type) and dcl3 mutant lines of Chlamydomonas reinhardtii
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Illumina HiSeq 2000
ORGANISM(S): Chlamydomonas reinhardtii
SUBMITTER: Bruno Santos
PROVIDER: E-MTAB-3852 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs.
Genome research 20160311 4
We describe here a forward genetic screen to investigate the biogenesis, mode of action, and biological function of miRNA-mediated RNA silencing in the model algal species,Chlamydomonas reinhardtii Among the mutants from this screen, there were three at Dicer-like 3 that failed to produce both miRNAs and siRNAs and others affecting diverse post-biogenesis stages of miRNA-mediated silencing. The DCL3-dependent siRNAs fell into several classes including transposon- and repeat-derived siRNAs as in ...[more]
