Transcriptome wide mapping of Hfq mediated RNA-RNA interactions in E. coli (RIL-seq)

ABSTRACT: Most E. coli sRNAs interact with their mRNA targets through simultaneous binding to the Hfq chaperon. In this experiment we cross-linked RNA to proteins in-vivo then did Hfq IP followed by ligation of bound RNAs and sequencing to identify sRNA-mRNA interactions. We termed the method RIL-seq for RNA Interactions by Ligation - sequencing.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Sahar Melamed 

PROVIDER: E-MTAB-3910 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress