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RNA-seq analysis of wild type Columbia seedlings

ABSTRACT: RNA was isolated from 14 day old Arabidopsis Col-0 seedlings in three biological repeats using the RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega). A Trueseq RNA-Seq library (Illumina) was compiled and sequenced as 50-bp single read using Illumina HiSeq 2000.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Plant Systems Biology data submission

PROVIDER: E-MTAB-3916 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The RING E3 Ligase KEEP ON GOING Modulates JASMONATE ZIM-DOMAIN12 Stability.

Pauwels Laurens L Ritter Andrés A Goossens Jonas J Durand Astrid Nagels AN Liu Hongxia H Gu Yangnan Y Geerinck Jan J Boter Marta M Boter Marta M Vanden Bossche Robin R De Clercq Rebecca R Van Leene Jelle J Gevaert Kris K De Jaeger Geert G Solano Roberto R Stone Sophia S Innes Roger W RW Callis Judy J Goossens Alain A

Plant physiology 20150828 2

Jasmonate (JA) signaling in plants is mediated by the JASMONATE ZIM-DOMAIN (JAZ) proteins that repress the activity of several transcription factors regulating JA-inducible gene expression. The hormone JA-isoleucine triggers the interaction of JAZ repressor proteins with the F-box protein CORONATINE INSENSITIVE1 (COI1), part of an S-phase kinase-associated protein1/Cullin1/F-box protein COI1 (SCF(COI1)) E3 ubiquitin ligase complex, and their degradation by the 26S proteasome. In Arabidopsis (Ara ...[more]

PMID: 26320228

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2-week-old Arabidopsis seedlings (Columbia ecotype)

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