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Analysis of single CD41 cell transcriptomes from Zebrafish

ABSTRACT: Transcriptome data from individual CD41 expressing cells isolated from kidney and heart of zebrafish. CD41 is a marker both for HSCs and thrombocytes, and with this data we study thrombocyte development as a continuous process. Single cell transcriptomes are matched with flourescence of a CD41:GFP reporter.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Danio rerio

SUBMITTER: Valentine Svensson

PROVIDER: E-MTAB-3947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-Cell RNA-Sequencing Reveals a Continuous Spectrum of Differentiation in Hematopoietic Cells.

Macaulay Iain C IC Svensson Valentine V Labalette Charlotte C Ferreira Lauren L Hamey Fiona F Voet Thierry T Teichmann Sarah A SA Cvejic Ana A

Cell reports 20160121 4

The transcriptional programs that govern hematopoiesis have been investigated primarily by population-level analysis of hematopoietic stem and progenitor cells, which cannot reveal the continuous nature of the differentiation process. Here we applied single-cell RNA-sequencing to a population of hematopoietic cells in zebrafish as they undergo thrombocyte lineage commitment. By reconstructing their developmental chronology computationally, we were able to place each cell along a continuum from s ...[more]

PMID: 26804912

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