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Early nutrient starvation response of the diatom Phaeodactylum tricornutum

ABSTRACT: An experiment was performed to determine the similarities on the RNA level between different conditions where cell division stops in the diatom Phaeodactylum tricornutum. Many of these conditions also increase the accumulation of lipids within the cell or impair photosynthesis. The different metabolic responses were evaluated and the dataset was mined for potential transcriptional regulators of these changes. The experimental setup was as follows: Cells from the pennate diatom Phaeodactylum tricornutum were grown in ESAW medium under continous fluorescent light at 21C in baffled shakeflasks. Exponentially growing cells were harvested by centrifugation and washed twice in 21gr/L NaCL to remove nutrients. Cells were subsequently resuspended in the five different media/conditions (control, darkness, no nitrate, no phosphate, nocodazole).

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Phaeodactylum tricornutum

SUBMITTER: Plant Systems Biology data submission

PROVIDER: E-MTAB-4096 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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