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Deep sequencing of human naive CD4 T cells identifies miR-34c-5p as a novel regulator of T cell activation and HIV replication

ABSTRACT: We generated a model for miR-34c-5p overexpression by transfecting Jurkat T cell line with a pcDNA3 vector containing the pre-miR-34c-5p precursor sequence and selecting for antibiotic resistance to isolate both pools and clones of stably transfected cells (J34c cells), in parallel with control cells transfected with the empty parental vector (JÃ cells). Both pools of stably transfected cells and the derived clonal lines expressed high levels of the miR.

ORGANISM(S): Homo sapiens

SUBMITTER: Andreia Amaral J.

PROVIDER: E-MTAB-4190 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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