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Arabidopsis thaliana strain:Col-0 Epigenomics

ABSTRACT: Cytosine DNA methylation (mC) is a genome modification that can regulate the expression of coding and non-coding genetic elements. However, little is known about the involvement of mC in response to environmental cues. We performed whole genome bisulfite sequencing to assess the spatio-temporal dynamics of mC in Arabidopsis grown under phosphate starvation.

INSTRUMENT(S): Illumina HiSeq 1000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: David Secco

PROVIDER: E-MTAB-4211 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Stress induced gene expression drives transient DNA methylation changes at adjacent repetitive elements.

Secco David D Wang Chuang C Shou Huixia H Schultz Matthew D MD Chiarenza Serge S Nussaume Laurent L Ecker Joseph R JR Whelan James J Lister Ryan R

eLife 20150721

Cytosine DNA methylation (mC) is a genome modification that can regulate the expression of coding and non-coding genetic elements. However, little is known about the involvement of mC in response to environmental cues. Using whole genome bisulfite sequencing to assess the spatio-temporal dynamics of mC in rice grown under phosphate starvation and recovery conditions, we identified widespread phosphate starvation-induced changes in mC, preferentially localized in transposable elements (TEs) close ...[more]

PMID: 26196146

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Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1); on Col-0 (Col-0/Col-0). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on the peat mix, leaf tissue was collected at the bolting and used for the bisulfite sequencing (methylome). Tissue from the msh1 mutant and dcl2,3,4,msh1 quadruple mutants used as rootstocks was similarly collected at the bolting stage and used for the bisulfite sequencing.

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2014-08-10 | E-MTAB-4391 | biostudies-arrayexpress

Mapping QTLs for 15 morpho-metric traits in <i>Arabidopsis thaliana</i> using Col-0 × Don-0 population.

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