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Fine kinetic transcriptome analysis during jasmonate signaling

ABSTRACT: To study the role of the plant hormone jasmonate in regulating stress-induced allocation of photosynthetic products between growth- and defense-related processes, we used RNA-sequencing to query the Arabidopsis transcriptome at high temporal resolution over 24 h after treatment with the bacterial toxin coronatine (COR, 5 micromolar), a high-affinity agonist of the JA receptor, or with a mock solution to account for diurnal changes in gene expression. These data establish a fine-scale view of the kinetics of jasmonate signaling, as well as of the diurnal patterns of gene expression.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Greg Howe

PROVIDER: E-MTAB-4218 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Temporal Dynamics of Growth and Photosynthesis Suppression in Response to Jasmonate Signaling.

Attaran Elham E Major Ian T IT Cruz Jeffrey A JA Rosa Bruce A BA Koo Abraham J K AJ Chen Jin J Kramer David M DM He Sheng Yang SY Howe Gregg A GA

Plant physiology 20140512 3

Biotic stress constrains plant productivity in natural and agricultural ecosystems. Repression of photosynthetic genes is a conserved plant response to biotic attack, but how this transcriptional reprogramming is linked to changes in photosynthesis and the transition from growth- to defense-oriented metabolism is poorly understood. Here, we used a combination of noninvasive chlorophyll fluorescence imaging technology and RNA sequencing to determine the effect of the defense hormone jasmonate (JA ...[more]

PMID: 24820026

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