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Expression analysis of Arabidopsis seedlings 3h and 2h after transfer to sucrose

ABSTRACT: Seedlings are grown on a mesh covering MS media without carbon source, and subsequently transferred at 9 DAS to control media without sucrose or medium supplemented with 15 mM sucrose. 3 hours and 24 hours after transfer, seedlings were harvested and the third leaf micro-dissected for RNA extraction.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Dirk Inze

PROVIDER: E-MTAB-4262 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Chloroplasts Are Central Players in Sugar-Induced Leaf Growth.

Van Dingenen Judith J De Milde Liesbeth L Vermeersch Mattias M Maleux Katrien K De Rycke Riet R De Bruyne Michiel M Storme Véronique V Gonzalez Nathalie N Dhondt Stijn S Inzé Dirk D

Plant physiology 20160301 1

Leaves are the plant's powerhouses, providing energy for all organs through sugar production during photosynthesis. However, sugars serve not only as a metabolic energy source for sink tissues but also as signaling molecules, affecting gene expression through conserved signaling pathways to regulate plant growth and development. Here, we describe an in vitro experimental assay, allowing one to alter the sucrose (Suc) availability during early Arabidopsis (Arabidopsis thaliana) leaf development, ...[more]

PMID: 26932234

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