Transcriptome profiling reveals regulatory and functional differentiation between maize mesophyll and bundle sheath cells

ABSTRACT: Maize, as a C4 plant, possesses two distinct types of photosynthetic cells, the mesophyll (M) and the bundle sheath (BS) cells. To elucidate their functional and regulatory differentiation we isolated large quantities of highly homogeneous M and BS cells from new mature leaves for transcriptome profiling by Illumina sequencing. More than 100,000,000 reads for each cell type were mapped to the maize genome, revealing 44,964 expressed genes. Among these, 37,105 genes were expressed in M cells, including 423 M-enriched genes and 20 M-enriched transcription factor (TF) genes, whereas 42,782 genes were expressed in BS cells, including 771 BS-enriched genes and 75 BS-enriched TF genes. Pathway analyses revealed cell differentiation in various cellular activities, with M cells playing more import

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Zea mays

SUBMITTER: Wen-Hsiung Li 

PROVIDER: E-MTAB-4320 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress