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Unknown,Transcriptomics,Genomics,Proteomics

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Characterization of transcriptional reprogramming of Vitis vinifera during Trichoderma-induced resistance and in response to Plasmopara viticola using RNA-Seq

ABSTRACT: We have carried out a global transcriptional analysis of grapevine resistance induced by Trichoderma harzianum T39 against Plasmopara viticola using high-throughput Illumina sequencing technology. Four samples were analyzed: control (C), T39-treated (T39), P. viticola-inoculated control (C+P.v.) and P. viticola-inoculated T39-treated (T39 P.v.) plants. Three biological replicates (named A, B and C) were analyzed for each sample and sequenced twice (named 1 and 2). Reads were aligned to the Pinot Noir ENTAV 115 genome and then analyzed to measure gene expression levels. Differentially expressed genes have been identified by statistical analysis.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Vitis vinifera

SUBMITTER: Michele Perazzolli

PROVIDER: E-MTAB-4324 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Downy mildew resistance induced by Trichoderma harzianum T39 in susceptible grapevines partially mimics transcriptional changes of resistant genotypes.

Perazzolli Michele M Moretto Marco M Fontana Paolo P Ferrarini Alberto A Velasco Riccardo R Moser Claudio C Delledonne Massimo M Pertot Ilaria I

BMC genomics 20121122

<h4>Background</h4>Downy mildew, caused by Plasmopara viticola, is one of the most severe diseases of grapevine and is commonly controlled by fungicide treatments. The beneficial microorganism Trichoderma harzianum T39 (T39) can induce resistance to downy mildew, although the molecular events associated with this process have not yet been elucidated in grapevine. A next generation RNA sequencing (RNA-Seq) approach was used to study global transcriptional changes associated with resistance induce ...[more]

PMID: 23173562

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