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RNA-directed DNA methylation in near-gene chromatin regulation

ABSTRACT: The purpose of this study was to measure DNA methylation and siRNA expression across the maize genome. The experimental data was derived from shotgun bisulfite sequencing, siRNA sequencing, and mRNA sequencing (Illumina, single end for all three)

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Zea mays

SUBMITTER: Kelly Dawe

PROVIDER: E-MTAB-4329 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CHH islands: de novo DNA methylation in near-gene chromatin regulation in maize.

Gent Jonathan I JI Ellis Nathanael A NA Guo Lin L Harkess Alex E AE Yao Yingyin Y Zhang Xiaoyu X Dawe R Kelly RK

Genome research 20121226 4

Small RNA-mediated regulation of chromatin structure is an important means of suppressing unwanted genetic activity in diverse plants, fungi, and animals. In plants specifically, 24-nt siRNAs direct de novo methylation to repetitive DNA, both foreign and endogenous, in a process known as RNA-directed DNA methylation (RdDM). Many components of the de novo methylation machinery have been identified recently, including multiple RNA polymerases, but specific genetic features that trigger methylation ...[more]

PMID: 23269663

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