Unknown,Transcriptomics,Genomics,Proteomics

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Oryza sativa cultivar:Nipponbare Transcriptome or Gene expression


ABSTRACT: A comprehensive time-course experiment of Pi-starved plants was undertaken, spanning medium (3 and 7 days), and long-term (21 days up to 52 days) Pi deprivation (−Pi), as well as both short term (1 and 3 days) and long-term (31 days) recovery. The 52 days time point consisting of 21 days starvation +31 days recovery enabled investigation of the effects of long term resupply on Pi starved plants, and coincided with the emergence of the first panicles and grains. Pre-germinated rice seedlings were grown for 14 days in Pi sufficient conditions (0.32 mM Pi) before being transferred to either Pi sufficient (0.32 mM Pi) or Pi deficient (0 mM Pi) media for 21 days. After 21 days of Pi deficient treatment, half of the plants were either maintained under Pi deficient conditions or re-supplied with Pi (0.32 mM) for 1, 3 or 31 days. To confirm the effectiveness of the Pi starvation and resupply treatments, physiological and molecular analyses were performed.

INSTRUMENT(S): Illumina HiSeq 1000

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: David Secco 

PROVIDER: E-MTAB-4334 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Stress induced gene expression drives transient DNA methylation changes at adjacent repetitive elements.

Secco David D   Wang Chuang C   Shou Huixia H   Schultz Matthew D MD   Chiarenza Serge S   Nussaume Laurent L   Ecker Joseph R JR   Whelan James J   Lister Ryan R  

eLife 20150721


Cytosine DNA methylation (mC) is a genome modification that can regulate the expression of coding and non-coding genetic elements. However, little is known about the involvement of mC in response to environmental cues. Using whole genome bisulfite sequencing to assess the spatio-temporal dynamics of mC in rice grown under phosphate starvation and recovery conditions, we identified widespread phosphate starvation-induced changes in mC, preferentially localized in transposable elements (TEs) close  ...[more]

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