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PolyA RNA sequencing of 7d old Arabidopsis thaliana seedlings

ABSTRACT: We performed transcriptome and ribosome immunoprecipitation studies of transgenic Arabidopsis (Arabidopsis thaliana) Columbia seeds expressing a His6FLAG-tagged version of the ribosomal large subunit protein L18B (35S:HF-RPL18B)

INSTRUMENT(S): Illumina Genome Analyzer

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Shin-Han Shiu 

PROVIDER: E-MTAB-4387 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Characteristics and significance of intergenic polyadenylated RNA transcription in Arabidopsis.

Moghe Gaurav D GD   Lehti-Shiu Melissa D MD   Seddon Alex E AE   Yin Shan S   Chen Yani Y   Juntawong Piyada P   Brandizzi Federica F   Bailey-Serres Julia J   Shiu Shin-Han SH  

Plant physiology 20121106 1

The Arabidopsis (Arabidopsis thaliana) genome is the most well-annotated plant genome. However, transcriptome sequencing in Arabidopsis continues to suggest the presence of polyadenylated (polyA) transcripts originating from presumed intergenic regions. It is not clear whether these transcripts represent novel noncoding or protein-coding genes. To understand the nature of intergenic polyA transcription, we first assessed its abundance using multiple messenger RNA sequencing data sets. We found 6  ...[more]

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