Unknown,Transcriptomics,Genomics,Proteomics

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Brachypodium distachyon, Bd21 RNA-Seq - Conserved Poaceae Specific Genes Project


ABSTRACT: The Poaceae family, also known as the grasses, includes agronomically important cereal crops such as rice, maize, sorghum, and wheat. Previous comparative studies have shown that much of the gene content is shared among the grasses; however, functional conservation of orthologous genes has yet to be explored. To gain an understanding of the genome-wide patterns of evolution of gene expression across reproductive tissues, we employed a sequence-based approach to compare analogous transcriptomes in species representing three Poaceae subgroups including the Pooideae (Brachypodium distachyon), the Panicoideae (sorghum), and the Ehrhartoideae (rice). Our transcriptome analyses reveal that only a fraction of orthologous genes exhibit conserved expression patterns. A high proportion of conserved orthologs include genes that are upregulated in physiologically similar tissues such as leaves, anther, pistil, and embryo, while orthologs that are highly expressed in seeds show the most diverged expression patterns. This experiment is related to E-MTAB-4400 (http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4400/) and E-MTAB-4402 (http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4402/)

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Brachypodium distachyon

SUBMITTER: Robin Buell 

PROVIDER: E-MTAB-4401 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Comparative transcriptomics of three Poaceae species reveals patterns of gene expression evolution.

Davidson Rebecca M RM   Gowda Malali M   Moghe Gaurav G   Lin Haining H   Vaillancourt Brieanne B   Shiu Shin-Han SH   Jiang Ning N   Robin Buell C C  

The Plant journal : for cell and molecular biology 20120605 3


The Poaceae family, also known as the grasses, includes agronomically important cereal crops such as rice, maize, sorghum, and wheat. Previous comparative studies have shown that much of the gene content is shared among the grasses; however, functional conservation of orthologous genes has yet to be explored. To gain an understanding of the genome-wide patterns of evolution of gene expression across reproductive tissues, we employed a sequence-based approach to compare analogous transcriptomes i  ...[more]

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