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Oryza sativa strain:Zhonghua11 Transcriptome or Gene expression

ABSTRACT: In the current study, we characterized an miRNA, OsmiR397, which was found to be associated with increased grain size, more rice panicle branching and higher grain productivity. We also elucidated the molecular mechanisms by which OsmiR397 increased grain yield. This miRNA downregulated the expression of its target gene, OsLAC, which then affected the sensitivity of plants to brassinosteroids. These results should be useful for breeding high-yield crops through genetic engineering. We performed RNA-seq on the young panicles of the wild-type, OXmiR397b and OXLAC plants and found that lots of brassinosteroid-related genes were differentially expressed between the three samples

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Yu-Chan Zhang

PROVIDER: E-MTAB-4429 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching.

Zhang Yu-Chan YC Yu Yang Y Wang Cong-Ying CY Li Ze-Yuan ZY Liu Qing Q Xu Jie J Liao Jian-You JY Wang Xiao-Jing XJ Qu Liang-Hu LH Chen Fan F Xin Peiyong P Yan Cunyu C Chu Jinfang J Li Hong-Qing HQ Chen Yue-Qin YQ

Nature biotechnology 20130721 9

Increasing grain yields is a major focus of crop breeders around the world. Here we report that overexpression of the rice microRNA (miRNA) OsmiR397, which is naturally highly expressed in young panicles and grains, enlarges grain size and promotes panicle branching, leading to an increase in overall grain yield of up to 25% in a field trial. To our knowledge, no previous report has shown a positive regulatory role of miRNA in the control of plant seed size and grain yield. We determined that Os ...[more]

PMID: 23873084

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