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Oryza sativa Transcriptome or Gene expression

ABSTRACT: In this study, we used RNA-Seq to understand the mechanisms of Cd toxicity, cellular detoxification and protection pathways in response to Cd in rice roots. To gain additional insight into the rice transcriptomic response to environmental Cd stress, 15-day-old rice seedlings were treated with 10 or 100 Î¼M solutions of Cd2+, or without Cd (control), for 24 h, at which point root samples were harvested and labeled as Cd+, Cd++, and control, respectively. These samples were used for 101 bp paired-end (PE) deep sequencing on an Illumina HiSeq 2500 platform.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Luqing Zheng

PROVIDER: E-MTAB-4445 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-Seq Analysis of Rice Roots Reveals the Involvement of Post-Transcriptional Regulation in Response to Cadmium Stress.

He Fei F Liu Qingquan Q Zheng Li L Cui Yaqiong Y Shen Zhenguo Z Zheng Luqing L

Frontiers in plant science 20151221

Widely-spread cadmium (Cd) pollution in the soil threatens both crop production and human health. How plants deal with the excess Cd are largely unknown. To evaluate the molecular mechanism by which plants respond to Cd stress, rice seedlings were treated with two concentrations of Cd and subjected to deep RNA sequencing. Comprehensive RNA-Seq analysis of rice roots under two gradients of Cd treatment revealed 1169 Cd toxicity-responsive genes. These genes were involved in the reactive oxygen sp ...[more]

PMID: 26734039

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