Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

MRNA time-course transcription profiling of bacteriocytes and gut tissue of pea aphids reared on standard artificial diet (AP3) and artificial diet depleted in Tyr and Phe (YFØ)


ABSTRACT: Tyrosine (Tyr, Y) and phenylalanine (Phe, F) synthesis is shared by the pea aphid and its symbiont Buchnera aphidicola.These aromatic amino acids are essential for the pea aphid growth and development. To characterize the molecular mechanisms, at gene transcriptional level, underlying this symbiotic integrated network pea aphids (Acyrthosyphon pisum, clone LL01) were reared on (i) standard artificial diet (AP3) and (ii) on the same AP3 medium depleted of Tyr (Y) and Phe (F). From each of the two groups, aphids were collected at specific time points and dissected: 12 h (D0), 1 day (D1), 2 days (D2), 3 days (D3), 4 days (D4), 5 days (D5) and 7 days (D7). Total RNA, to be used in gene expression analysis by arrays, was extracted, under the two rearing conditions, from two tissues: gut [from 20 aphids per sample at all 7 time points] and bacteriocytes [from 25 aphids per sample at 4 time points: 3 days (D3), 4 days (D4), 5 days (D5) and 7 days (D7)]. At each time point we included three biological replicates.

ORGANISM(S): Acyrthosiphon pisum

SUBMITTER: Stefano Colella 

PROVIDER: E-MTAB-4456 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2016-12-14 | PXD004893 | Pride
2021-12-01 | E-MTAB-10540 | biostudies-arrayexpress
2014-06-04 | E-GEOD-56830 | biostudies-arrayexpress
2010-05-14 | E-GEOD-18658 | biostudies-arrayexpress
2010-05-14 | E-GEOD-18657 | biostudies-arrayexpress
2006-03-15 | E-TABM-83 | biostudies-arrayexpress
2012-01-31 | E-GEOD-35427 | biostudies-arrayexpress
2018-10-10 | E-MTAB-5228 | biostudies-arrayexpress
2020-11-09 | PXD010663 | Pride
2011-09-13 | E-GEOD-31024 | biostudies-arrayexpress