Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mating system manipulation and the evolution of sex-biased gene expression in Drosophila.

ABSTRACT: Exploring gene expression changes in samples of D. pseudoobscura after 157 generations of experimental monogamy or elevated polyandry. Two tissues (abdomens, heads), two sexes, two courtship statuses (virgin, courted) and 3 biological treatments (Baseline, Elevated promiscuity, Monogamy).

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Drosophila pseudoobscura bogotana

SUBMITTER: Yongxiang Fang

PROVIDER: E-MTAB-4486 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

D pseudoobscura reproductive tissue RNAseq after experimental sexual selection

Project description:RNA from virgin male and virgin or mated females from the same or different treatment (monogamous, polyandrous)

2022-04-02 | E-MTAB-10047 | biostudies-arrayexpress

Transcriptional profiles of experimentally evolved monogamous and polygamous Drosophila melanogaster

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-03-17 | E-GEOD-50918 | biostudies-arrayexpress

Sex-biased expression in Drosophila willistoni

Project description:We performed mRNA sequencing of samples isolated from the heads, thoraxes, and abdomens of males and females of Drosophila willistoni to identify genes that are differentially expressed between the sexes. Comparison of expression levels in females and males

2012-04-08 | E-GEOD-31723 | biostudies-arrayexpress

Transcriptional profiling of Drosophila melanogaster heads from experimentally-evolved populations

Project description:We used RNA sequencing to examine the transcriptomes of male and female heads from experimentally-evolved D. melanogaster populations after 117 generations of mating system manipulation in order to examine the pattern of evolution in sex-biased genes. Examined head transcriptomes of 3 monogamous populations and 3 polygamous populations, both males and females, for 12 total samples.

2014-03-17 | E-GEOD-50915 | biostudies-arrayexpress

Post-mating expression of female Drosophila melanogaster genomes experimentally evolved under different post-copulatory sexual selection regimes

Project description:By combining an experimental evolution approach with genomic techniques, we investigated the effects of seminal fluid on female gene expression. In our study, we experimentally manipulated the mating system in replicate populations of D. melanogaster, by removing post-copulatory sexual selection, with the aim of testing differences in short term post-mating reaction of females evolved under different mating strategies. We show that monogamous females suffer decreased fecundity, regardless of the type of male they were mated with, and that their post-mating gene expression profiles differ significantly from promiscuous females, involving 1141 transcripts (9% of the genes tested). These transcripts are active in several tissues, mainly ovaries, neural tissues, midgut and spermathecae, and are involved in metabolic processes, reproduction and signaling pathways. Our results provide a list of candidate genes responsible for the decrease in female fecundity in the absence of post-copulatory sexual selection, and demonstrate how the female post-mating response can evolve under different mating systems over relatively short time frames. From an LHM base population, we created 8 replicate populations and maintained them under experimental evolution: 4 populations were allowed to mate only once every generation (monogamy), and the other 4 were kept under the standard mating protocol (promiscuous). After 46 generations, we crossed males and females within the same population and with individuals of the opposite treatment. Mated female flies were frozen 6 h after mating and RNA extracted. Two biological replicates per cross per population (2x2x8=32 samples).

2014-01-01 | E-GEOD-48385 | biostudies-arrayexpress

Expression analysis of evolved polygamous and monogamous Drosophila melanogaster

Project description:Investigation of gene expression level changes in evolved polygamous and monogamous populations of Drosophila melanogaster. The populations investigated are described in Hollis et al. 2011. Populations with elevated mutation load do not benefit from the operation of sexual selection. Journal of Evolutionary Biology 24: 1918-1926. A study using total RNA extracted from male and female virgin 4-day old Drosophila melanogaster and then transcriptionally profiled with 12x135k Nimblegen arrays. Also, transcriptional profiling of male and female heads from the same populations using Illumina RNA-Seq.

2014-03-17 | E-GEOD-39742 | biostudies-arrayexpress

16S Metagenomics of mosquitoes

Project description:16S Metagenomics of mosquito abdomens

| PRJEB32570 | ENA

RNA-seq of achilles tendon from young and old donors to study age-related changes in tendon

Project description:Tendon from young and old donors was used for RNA-Seq analysis. The aim of the study was to identify differentially expressed tendon transcripts in ageing in order to to characterize molecular mechanisms associated with age-related changes in tendon.

2015-04-01 | E-MTAB-2449 | biostudies-arrayexpress

Sex-biased expression in Drosophila willistoni

2012-04-08 | GSE31723 | GEO

Sex-, stage-, and tissue-specific alternative splicing in Drosophila melanogaster

Project description:We used long-oligonucleotide microarrays to investigate whether alternative splicing in Drosophila is regulated in a sex-, stage-, or tissue-specific manner. To examine sex-specific splicing, we compared gene expression profiles of male and female pupae 12 hours after pupariation. To examine stage-specific splicing, we compared expression profiles of mixed-sex, 0-24 hour old embryos and mixed-sex, 12 hour old pupae. To examine tissue-specific splicing, we compared expression profiles of adult male heads and abdomens 24-48 hours after eclosion. To examine tissue-specific splicing, we compared expression profiles of adult male heads and abdomens at 24-48 hours after eclosion. Keywords: tissue-specific expression profiles

2007-06-22 | GSE5190 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data