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Regulation of microRNA-mediated developmental changes by the SWR1 chromatin remodeling complex in Arabidopsis thaliana

ABSTRACT: We investigated small-RNA populations from Arabidopsis thaliana Col-0 between Wild-type and the arp6 mutant with focus on micro-RNA levels. Small-RNA sequencing was performed for 4 bioreps (with 4 technical replicates each) of each condition. Size selection was done by manually cutting bands.

INSTRUMENT(S): growth chamber, NextSeq 500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Kyuha Choi

PROVIDER: E-MTAB-4498 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of MicroRNA-Mediated Developmental Changes by the SWR1 Chromatin Remodeling Complex.

Choi Kyuha K Kim Juhyun J Müller Sebastian Y SY Oh Mijin M Underwood Charles C Henderson Ian I Lee Ilha I

Plant physiology 20160418 2

The ATP-dependent SWR1 chromatin remodeling complex (SWR1-C) exchanges the histone H2A-H2B dimer with the H2A.Z-H2B dimer, producing variant nucleosomes. Arabidopsis thaliana SWR1-C contributes to the active transcription of many genes, but also to the repression of genes that respond to environmental and developmental stimuli. Unlike other higher eukaryotic H2A.Z deposition mutants (which are embryonically lethal), Arabidopsis SWR1-C component mutants, including arp6, survive and display a plei ...[more]

PMID: 27208270

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