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Meiotic crossover hotspots and coldspots within Arabidopsis disease resistance genes

ABSTRACT: To study recombination at the fine-scale we used high-throughput sequencing to analyse >1,000 crossovers within the RAC1 R gene hotspot. This revealed focused intragenic crossovers, overlapping exons encoding the TIR, NBS and LRR domains (RAC1 pollen typing sequencing). To analyse chromatin structure we performed micrococcal nuclease digestion of wild type (Col-0) chromatin and gel purified the resulting ~150 bp mononucleosomal DNA band. This DNA was used to generate a library and paired-end sequencing performed (MNase-seq)

INSTRUMENT(S): Illumina MiSeq, Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ian Henderson

PROVIDER: E-MTAB-4556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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