Project description:The first 4 samples belong to the RNA-IP using in situ TAP tagged ZC3H30 in procyclic (insect) form of the parasite T. brucei Lister 427, 2 samples are Elu or eluate, and 2 are FL or flowthrough (unbound) sample. The other 8 samples are also from procyclic cells. 4 samples belong to DKO(ZC3H30 gene double knockout), 2 are non-stressed and 2 are heat shocked samples; the rest 4 samples are DKO-ectopic (ZC3H30 double knockouts, expressing, ectopic copy of ZC3H30) 2 are non-stressed and 2 are heat shocked samples. Heat Shock experiment was done at 39 degree Celsius.

2017-12-16 | E-MTAB-6281 | biostudies-arrayexpress

RBP9-TAP RIPseq in Trypanosoma brucei

Project description:RBP9-TAP was expressed in bloodstream T. brucei and bound mRNA was pulled down using IgG beads and eluted via TEV-protease cleavage. rRNA was depleted using RNAseH and rRNA hybridizing oligos.

2020-05-30 | E-MTAB-9093 | biostudies-arrayexpress

RNA-IP of TAP-tagged 4EIP in Trypanosoma brucei

Project description:RNA-IP of TAP-tagged 4EIP in Trypanosoma brucei

| PRJEB23807 | ENA

RNAseq of DRBD7-TAP interacting RNAs in Trypanosoma brucei

Project description:DRBD7 interacting mRNAs were identified using a DRBD7-TAP fusion protein for protein pulldown. To study enrichment, the flow-through was also subjected to RNAseq.

2020-05-30 | E-MTAB-9092 | biostudies-arrayexpress

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