Unknown,Transcriptomics,Genomics,Proteomics

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DEG9, a serine protease modulates cytokinins and light signaling by regulating the level of Arabidopsis Response Regulator 4


ABSTRACT: The Arabidopsis seedlings treated with 2M t-zeatin (CTK) for 30 minutes and then total RNA was extracted from Arabidopsis seedlings using TRIzol reagent (Invitrogen) and treated with RNase-free DNase I. The poly(A) mRNA was isolated using oligo (dT) beads. First-strand cDNA was subsequently generated using random hexamer-primed reverse transcription, followed by synthesis of the second-strand cDNA using RNaseH and DNA polymerase I. Then, single-end and paired-end RNA-seq libraries were prepared following Illumina's protocols and sequenced on the Illumina GA II platform.

INSTRUMENT(S): Agilent 2100 Bioanaylze, Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Wei Chi 

PROVIDER: E-MTAB-4603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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