Unknown,Transcriptomics,Genomics,Proteomics

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Transcription analyses of the tic56-1 import mutant line


ABSTRACT: In order to distinguish transcriptional from posttranscriptional effects on plastid proteome assembly we performed GeneChip analyses with total RNA of 8 week old tic56-1 plants and compared the data with wildtype treated in parallel.

INSTRUMENT(S): SureScan Microarray Scanner (Agilent Technologies)

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Baginsky Sacha 

PROVIDER: E-MTAB-4650 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Importance of Translocon Subunit Tic56 for rRNA Processing and Chloroplast Ribosome Assembly.

Köhler Daniel D   Helm Stefan S   Agne Birgit B   Baginsky Sacha S  

Plant physiology 20161012 4


Toc159-containing complexes at the outer chloroplast envelope membrane form stable supercomplexes with a 1-MD translocon at the inner chloroplast envelope membrane of which Tic56 is one essential subunit. While the single mutants tic56-1 and ppi2 (toc159) have an albino phenotype and are able to grow heterotrophically, we find the double mutant to be embryo lethal. Comprehensive quantitative proteome profiling with both single mutants in combination with GeneChip analyses identified a posttransc  ...[more]

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