Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Identification of Acute megakaryoblastic leukemia (AMKL) deregulated genes by RNAseq

ABSTRACT: Acute megakaryoblastic leukemia (AMKL) is a heterogeneous disease generally associated with poor prognosis. Gene expression profiles indicate the existence of distinct molecular subgroups, and several genetic alterations have been characterized in the past years, including the t(1;22)(p13;q13) and the trisomy 21 associated with GATA1 mutations. However, the majority of patients do not present known mutations, and the limited access to primary patient leukemic cells impedes the efficient development of novel therapeutic strategies. In this study, using a xenotransplantation approach, we have modeled human pediatric AMKL in immunodeficient mice. Analysis of high-throughput RNA sequencing identified recurrent fusion genes defining new molecular subgroups.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas MERCHER 

PROVIDER: E-MTAB-4697 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Chimeric transcription factors are a hallmark of human leukemia, but the molecular mechanisms by which they block differentiation and promote aberrant self-renewal remain unclear. Here, we demonstrate that the ETO2-GLIS2 fusion oncoprotein, which is found in aggressive acute megakaryoblastic leukemia, confers megakaryocytic identity via the GLIS2 moiety while both ETO2 and GLIS2 domains are required to drive increased self-renewal properties. ETO2-GLIS2 directly binds DNA to control transcriptio  ...[more]

Similar Datasets

Transcriptomics IGR_MERCHER_LAM7
2012-11-17 | E-MTAB-1370 | biostudies-arrayexpress
Transcriptomics Runx1 (Aml1) knockdown in AMkL Meg-01 cells
2009-08-01 | E-GEOD-17311 | biostudies-arrayexpress
Transcriptomics Gene expression profiling of Down Syndrome (DS)-AMKL and non-DS AMKL samples
2010-08-01 | GSE16677 | GEO
Genomics Identification of ETO2-GLIS2 targets by ChIP-sequencing
2017-05-22 | E-MTAB-4367 | biostudies-arrayexpress
Transcriptomics Gene expression profiling of Down Syndrome (DS)-AMKL and non-DS AMKL samples
2010-08-01 | E-GEOD-16677 | biostudies-arrayexpress
Transcriptomics RNA-SEQ Quantitative Analysis of WT and Mpl-del-overexpressing AMKL cell Transcriptomes
2019-04-04 | GSE127762 | GEO
Transcriptomics Mouse Models of Pediatric Acute Megakaryoblastic Leukemia Reveal Novel Therapeutic Vulnerabilities [DSP798]
2023-08-04 | GSE208157 | GEO
Transcriptomics Mouse Models of Pediatric Acute Megakaryoblastic Leukemia Reveal Novel Therapeutic Vulnerabilities [DSP750]
2023-08-04 | GSE208186 | GEO
Transcriptomics caArray_orkin-0038: Identification of distinct molecular phenotypes in acute megakaryoblastic leukemia by gene expression profiling
2016-06-17 | GSE83449 | GEO
Transcriptomics Mouse Models of Pediatric Acute Megakaryoblastic Leukemia Reveal Novel Therapeutic Vulnerabilities [DSP601-649-749]
2023-08-04 | GSE208158 | GEO