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Transcriptomic analysis of lactate dehydrogenase negative mutant of Enterococcus faecalis V583

ABSTRACT: In order to study the effect of lactate dehydrogenase (?ldh 1.2) mutant on the transcriptome DNA microarray technology was used. Stains were grown in a chemically defined medium (CDM-LAB) containing 1.1% of glucose as a carbon sources at 37?C on anaerobic condition to an optical density of 0.6.

ORGANISM(S): Enteroccus faecalis V583

SUBMITTER: Ibrahim Mehmeti

PROVIDER: E-MTAB-472 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome, proteome, and metabolite analyses of a lactate dehydrogenase-negative mutant of Enterococcus faecalis V583.

Mehmeti Ibrahim I Jönsson Maria M Fergestad Ellen M EM Mathiesen Geir G Nes Ingolf F IF Holo Helge H

Applied and environmental microbiology 20110204 7

A constructed lactate dehydrogenase (LDH)-negative mutant of Enterococcus faecalis V583 grows at the same rate as the wild type but ferments glucose to ethanol, formate, and acetoin. Microarray analysis showed that LDH deficiency had profound transcriptional effects: 43 genes in the mutant were found to be upregulated, and 45 were found to be downregulated. Most of the upregulated genes encode enzymes of energy metabolism or transport. By two-dimensional (2D) gel analysis, 45 differentially expr ...[more]

PMID: 21296946

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