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Comprehensive transcriptome profiling in tomato using massively parallel pyro sequencing technology

ABSTRACT: Deep transcriptome sequencing of various tomato tissues. We describe the coupling of pyrosequencing technology with laser capture microdissection to characterize the transcriptomes of the five principal tissues of the pericarp from tomato fruits (outer and inner epidermal layers, collenchyma, parenchyma, and vascular tissues) at their maximal growth phase. NOTE: Not all samples from SRP004923 were imported in this accession.

INSTRUMENT(S): 454 GS FLX

ORGANISM(S): Solanum lycopersicum

SUBMITTER: Jocelyn Rose

PROVIDER: E-MTAB-4765 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tissue- and cell-type specific transcriptome profiling of expanding tomato fruit provides insights into metabolic and regulatory specialization and cuticle formation.

Matas Antonio J AJ Yeats Trevor H TH Buda Gregory J GJ Zheng Yi Y Chatterjee Subhasish S Tohge Takayuki T Ponnala Lalit L Adato Avital A Aharoni Asaph A Stark Ruth R Fernie Alisdair R AR Fei Zhangjun Z Giovannoni James J JJ Rose Jocelyn K C JK

The Plant cell 20111101 11

Tomato (Solanum lycopersicum) is the primary model for the study of fleshy fruits, and research in this species has elucidated many aspects of fruit physiology, development, and metabolism. However, most of these studies have involved homogenization of the fruit pericarp, with its many constituent cell types. Here, we describe the coupling of pyrosequencing technology with laser capture microdissection to characterize the transcriptomes of the five principal tissues of the pericarp from tomato f ...[more]

PMID: 22045915

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