Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Rapid reprogramming of primary human astrocytes into potent tumor initiating cells with defined genetic factors


ABSTRACT: Cancer stem cells play key roles in human malignancies. However, their cellular and molecular origins are still poorly understood. Here we report the successful generation of cancer stem cells from primary human astrocytes by use of defined genetic factors. Combined transduction of four factors —myc, Oct-4, p53DD, and ras— induced efficient transformation of primary human astrocytes into malignant cells with powerful tumor-initiating capabilities. Transplantation of 100 of the cells into nude mice is sufficient for tumor formation. The cells also showed unlimited self-renewal ability with robust telomerase activities. In addition, they expressed typical glioma stem cell markers such as CD133, CD15, and CD90. Moreover, transformed cells could form spheres in culture and differentiate into neuron-astrocyte-, and oligodendrocyte-like cells. They also displayed significant resistance to temozolomide. These induced cancer stem cells (iCSCs) should be useful for both tumor biology studies and therapeutics development. This record contains data from microarray analysis of normal human astrocytes, two transduced cell lines (OCT4+3G and OCT4+6G), a patient-derived glioma stem cell line (ALPS1459) and a glioblastoma cell line (U87MG).

ORGANISM(S): Homo sapiens

SUBMITTER: Chuan-Yuan Li 

PROVIDER: E-MTAB-4771 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Rapid Reprogramming of Primary Human Astrocytes into Potent Tumor-Initiating Cells with Defined Genetic Factors.

Li Fang F   Liu Xinjian X   Sampson John H JH   Bigner Darell D DD   Li Chuan-Yuan CY  

Cancer research 20160630 17


Cancer stem-like cells (CSC) are thought to drive brain cancer, but their cellular and molecular origins remain uncertain. Here, we report the successful generation of induced CSC (iCSC) from primary human astrocytes through the expression of defined genetic factors. Combined transduction of four factors, Myc, Oct-4, p53DD, and Ras, induced efficient transformation of primary human astrocytes into malignant cells with powerful tumor-initiating capabilities. Notably, transplantation of 100 transd  ...[more]

Similar Datasets

2013-01-05 | E-GEOD-42908 | biostudies-arrayexpress
2013-12-01 | E-GEOD-36145 | biostudies-arrayexpress
2009-07-06 | E-TABM-706 | biostudies-arrayexpress
2015-06-23 | E-GEOD-60388 | biostudies-arrayexpress
2012-07-13 | E-GEOD-29317 | biostudies-arrayexpress
2021-03-23 | E-MTAB-10030 | biostudies-arrayexpress
2021-10-05 | PXD026776 | Pride
2017-05-02 | E-MTAB-5514 | biostudies-arrayexpress
2018-08-08 | E-MTAB-5987 | biostudies-arrayexpress
2010-06-08 | E-GEOD-21799 | biostudies-arrayexpress