Unknown,Transcriptomics,Genomics,Proteomics

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Characterization of long non-coding RNAs expressed during oocyte-to-embryo transition in mice


ABSTRACT: Sequencing of polyA RNA fraction from MT oocytes and 1cell embryos.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Mus musculus

SUBMITTER: Vedran Franke 

PROVIDER: E-MTAB-4775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Long non-coding RNA exchange during the oocyte-to-embryo transition in mice.

Karlic Rosa R   Ganesh Sravya S   Franke Vedran V   Svobodova Eliska E   Urbanova Jana J   Suzuki Yutaka Y   Aoki Fugaku F   Vlahovicek Kristian K   Svoboda Petr P  

DNA research : an international journal for rapid publication of reports on genes and genomes 20170401 2


The oocyte-to-embryo transition (OET) transforms a differentiated gamete into pluripotent blastomeres. The accompanying maternal-zygotic RNA exchange involves remodeling of the long non-coding RNA (lncRNA) pool. Here, we used next generation sequencing and de novo transcript assembly to define the core population of 1,600 lncRNAs expressed during the OET (lncRNAs). Relative to mRNAs, OET lncRNAs were less expressed and had shorter transcripts, mainly due to fewer exons and shorter 5' terminal ex  ...[more]

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