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ChIP-seq analysis of H3K9me3 levels across the genome for one copy of H3K9M mutant in pombe

ABSTRACT: We performed ChIP-seq analyses for WT, H3K9M mutant and clr4 mutant in Schizosaccharomyces pombe, and showed that H3K9me3 level was reduced to background levels across the entire genome, similar to clr4 mutant.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Chunmin Shan

PROVIDER: E-MTAB-4776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A histone H3K9M mutation traps histone methyltransferase Clr4 to prevent heterochromatin spreading.

Shan Chun-Min CM Wang Jiyong J Xu Ke K Chen Huijie H Yue Jia-Xing JX Andrews Stuart S Moresco James J JJ Yates John R JR Nagy Peter L PL Tong Liang L Jia Songtao S

eLife 20160920

Histone lysine-to-methionine (K-to-M) mutations are associated with multiple cancers, and they function in a dominant fashion to block the methylation of corresponding lysines on wild type histones. However, their mechanisms of function are controversial. Here we show that in fission yeast, introducing the K9M mutation into one of the three histone H3 genes dominantly blocks H3K9 methylation on wild type H3 across the genome. In addition, H3K9M enhances the interaction of histone H3 tail with th ...[more]

PMID: 27648579

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