Transcriptomic response to concentrated pea root exudate

ABSTRACT: The secretion of metabolites by plant roots is a key determinant of microbial growth and colonisation. We have used Pisum sativum and its natural symbiont Rhizobium leguminosarum (it can form N2 fixing nodules on pea roots) to study the natural metabolites secreted by roots. To do this root secretion was harvested from pea plants grown under sterile conditions. This root exudate was then concentrated and used as a sole carbon and nitrogen source for growth of the bacteria in the laboratory. These bacteria were harvested in mid-exponential growth and RNA extracted for microarray analysis. As control cultures the bacteria were grown on 30 mM pyruvate as a carbon source and 10 mM ammonium chloride as a nitrogen source and RNA extracted. Two colour microarrays were performed using root exudate cultures versus pyruvate ammonia grown cultures. This was done in biological triplicate.

INSTRUMENT(S): Agilent Scanner

ORGANISM(S): Rhizobium leguminosarum

SUBMITTER: philip Poole 

PROVIDER: E-MTAB-4790 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress