Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Solanum lycopersicum Transcriptome or Gene expression

ABSTRACT: Lines nearly isogenic for fw3.2 in the cultivated background Solanum lycopersicum c.v. Yellow Stuffer were grown in the greenhouse in a completely randomized design. fw3.2(ys) and fw3.2(wt) are NILs carrying cultivated and wild alleles for fw3.2 locus. Flowers were tagged a day before anthesis and self-pollinated at anthesis. Fruits at stage five, seven, and ten days post anthesis (dpa) were harvested. Pericarp and seed tissues were separately isolated. Four replicates were collected for each sample. RNA was extracted using Trizol and Stand-specific libraries were prepared from total RNA and sequences of 51 bp were generated on an Illumina HiSeq2000.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Solanum lycopersicum

SUBMITTER: Esther van der Knaap

PROVIDER: E-MTAB-4792 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A cytochrome P450 regulates a domestication trait in cultivated tomato.

Chakrabarti Manohar M Zhang Na N Sauvage Christopher C Muños Stéphane S Blanca Jose J Cañizares Joaquin J Diez Maria Jose MJ Schneider Rhiannon R Mazourek Michael M McClead Jammi J Causse Mathilde M van der Knaap Esther E

Proceedings of the National Academy of Sciences of the United States of America 20130930 42

Domestication of crop plants had effects on human lifestyle and agriculture. However, little is known about the underlying molecular mechanisms accompanying the changes in fruit appearance as a consequence of selection by early farmers. We report the fine mapping and cloning of a tomato (Solanum lycopersicum) fruit mass gene encoding the ortholog of KLUH, SlKLUH, a P450 enzyme of the CYP78A subfamily. The increase in fruit mass is predominantly the result of enlarged pericarp and septum tissues ...[more]

PMID: 24082112

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Dataset Information

Solanum lycopersicum Transcriptome or Gene expression

Publications

A cytochrome P450 regulates a domestication trait in cultivated tomato.

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