Microarray analysis of HCT116 cells with and without a 1.4kb deletion

ABSTRACT: One of the strongest associated type 2 diabetes (T2D) loci reported to date resides within the TCF7L2 gene. Previous studies point to the T allele of rs7903146 in intron 3 as the causal variant at this locus. To aid in the identification of the actual gene(s) under the influence of this variant, we first generated a CRISPR/Cas9 mediated 1.4kb deletion of the genomic region harboring rs7903146 in the HCT116 cell line followed by global gene expression analysis. HCT116 cells with or without a CRISPR/Cas9 mediated1.4kb deletion of the genomic region harboring the SNP rs7903146 were analyzed for expression, with 3 replicates per condition (DEL vs WT). We observed 99 genes with significant differential expression (FDR cut-off=10%) and an effect size of at least two-fold. We then carried out hig

ORGANISM(S): Homo sapiens

SUBMITTER: Qianghua Xia 

PROVIDER: E-MTAB-4839 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress