Genome wide mapping of long range contacts unveils DNA Double Strand Breaks clustering at damaged active genes.

ABSTRACT: Translocations, that occur when two DNA Double Strand breaks (DSBs) are abnormally rejoined, represent highly deleterious genome rearrangements favoring cancer apparition and progression. However, the mechanisms that drive their formation are yet poorly deciphered. One prerequisite for translocation is the juxtaposition of two distant DSBs, an event that would be favored if DSB cluster, i.e. are brought together in close spatial proximity within the nucleus. Whether DSB cluster in higher eukaryotes has been subjected to a strong controversy over the past decade, due to conflicting results obtained using microscopy based methods1-9. Here we used for the first time a high throughput chromosome conformation capture assay (Capture Hi-C10) to investigate DSB clustering. We unambiguously found

INSTRUMENT(S): Illumina HiSEq 2500, Illumina HiSeq 1000

ORGANISM(S): Homo sapiens

SUBMITTER: Gaelle Legube 

PROVIDER: E-MTAB-4846 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress